• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    A dilutor/dispenser system wherein a marker, e.g., an air bubble, is introduced at the probe inlet prior to each aspiration cycle, so as to be displaced along the probe system during such cycle. The location of the marker is detected at the end of the aspiration cycle, to indicate whether an appropriate liquid volume has been aspirated. The aspirated liquid, along with the marker and a predetermined volume of diluent, is subsequently dispensed. An alarm may be activated, if an improper volume has been aspirated.
    公开号:SU795518A3
    申请号:SU782677800
    申请日:1978-10-02
    公开日:1981-01-07
    发明作者:М.Неджерсмит Кент
    申请人:Текникон Инструментс Корпорейшн(Фирма)；
    IPC主号:
    专利说明:

    (54) DEVICE FOR MIXING AND DOSING OF SEVERAL ADDED TASKS REAGENTS The purpose of the invention is to improve the reliability of the device in operation. This goal is achieved by providing the device with a marker insertion mechanism, made of a pillow and a hammer with a spring and a solenoid, and a detector tracking the marker location, and the connector on the tube is made with a section of elastic material with an elastic convex part located between the pillow and gavel. FIG. 1 and 2 show a solvent dosing device; in fig. 3 7 illustrates the operation of the device. The invention is a mixing and metering device comprising a tube 1 connected at one end with a probe 2 and at the other end with one of the channels of a three channel two-way valve 3. At least the input end of the probe 2 is made of a transparent material. The piston pump 4 of the sample is connected to the tube 1. The piston pump 5 of the reagent is connected to the second channel of the valve 3. The motors b and 7 are connected to the pump 4 of the sample and to the pump 5 of the reagent, respectively. The remaining channel of the valve 3 is connected through the tube 8 to the source 9 of the solvent. The described part of the design is common to many types of previously known devices for mixing and metering. Many receivers 10 sample. containing fluid samples to be analyzed and a plurality of cells 11 in which the reaction takes place are arranged in alternating order on turntable 12. Turntable 12 is mounted for removal on shaft 13 supported by bearing 14, mounted on horizontal plate 15 located within the housing 16. On the lower surface of the plate 15, an engine 17 is mounted, which is mechanically connected through this plate to a driving pin 18, mounted for engagement with the Maltese cross 19, connected with the shaft 13 in such a way as to turn the turntable 12 in the direction indicated by the arrow. The plate 15 is also adapted to be lifted or lowered by means of a worm gear 20, mechanically connected to a reversible motor 21 fixed on the bottom wall 22 of the housing 16. The worm gear 20 engages an element 23 having an internal cut bu rigidly mounted on plate 15. The removed edge of the plate is capable of receiving guide rods 24, the ends of which are located in the upper and lower walls 25 and 22 of the housing 16. As will be described later, the motor 17 is controlled to index the position of the swivel part 12 in such a way as to locate the receiver 10 or cell 11 directly under the probe 2. The reversible motor 21 is controlled between successive positions of the turntable 12 so as to position the input end of the probe 2 within the receiver 10 (during the cycle suction) or cells 11 (during the suction cycle). The operation of the device occurs with the help of the programmer 26. Before the beginning of the pa. Technician bots load a lot of sample receivers 10, each of which contains a sample of liquid to be analyzed, and cells 11 that respond to turntable 12. Such loading can be done while the turntable is in the lower position, or when it is removed from the shaft 13. The cell 11 is adapted to perform private work on the samples contained in the receiver 10 and the feed. Directly to probe 2. Each well contains the necessary reagent 27, which can be lyophilized to perform the desired analysis of the fluid sample. When the turntable 12 is loaded and in line with the receiver 10, located below the probe 2, the technician commands the programmer 26. The programmer 26 drives the reversible motor 21 to rotate the threaded element 23 so as to raise the turntable 12 and immerse the probe 2 in the fluid sample contained in the receiver 10. While the probe 2 is immersed, the programmer 26 commands the drive motor 6 to turn on the pump 4 and suction a predetermined sample volume through the probe 2 and tube 1. The sucked sample volume a is equal to the displacement of this pump, but not sufficient to completely fill the tube 1, as a result of which the pump 4 remains filled with solvent. At the same time, the pump 5 is filled with a solvent. Following the suction cycle, the programmer 26 issues a command to the engine 21 to work in the opposite direction to lower the rotary table 12 and remove the probe 2 from the receiver 10. When the probe 2 is removed, the programmer 26 drives the engine 17, which drives the pinion wheel 18 into engagement with a Maltese cross 19. and rotates the turntable 12 to place the neighboring cell 11 under the probe 2. When the cell 11 is placed under the probe 2, the programmer 26 commands the reversible motor 21 to lift the turntable 12 such
    This means that probe 2 passes through cover 28 and is located inside this cell. The cover 28 serves to seal the cell 11 and, as a rule, has a multi-layer construction, with one layer containing an absorbent layer for wiping the external surfaces of the probe 2 in order to prevent contamination between successive sampling. Programmer 26 sequentially drives motors b and 7 to return pump 4 and pump 5 in such a way as to dose the liquids contained in tube 1 and probe 2 into cell 11. The amount of fluid, t, e., Sample and solvent, dosed in the cell 11, the combined and controlled forward displacements of the sample pump 4 and the solvent pump 5 are equal. Thus, predetermined volumes of both the sample and the solvent are introduced into the well for reaction with the lyophilized reagent 27. The reactive mixture can be sequentially analyzed, for example, calorimetrically. Following the dosing cycle, the valve 3 is controlled by the programmer 26 to connect the solvent pump 5 through the tube 8 to the solvent source 9. At the same time, the programmer 26 actuates the drive motor 7 to remove the pump 5 so that the solvent is sucked and ready for the next operation. Valve 3 is sequentially brought back to normal by programmer 26, as indicated by the dotted line. To define a short sample, section 29 of tube 1 is formed from a flexible elastic material and defines an elastic convex part. The convex part of the tube section 29 is located between the pad 30 and the hammer 31. The lever 32 fixed at one end is rotatably connected at the other end by the protrusion 33 of the hammer 31. Typically the lever 32 is offset by the spring 34 to the lowered hammer 31, as shown in FIG. . 2. The lever 32 is mechanically connected to the solenoid plunger 35. Immediately prior to each suction cycle, the programmer 26 energizes the solenoid 35 to lift the hammer 31, whereby the elastic part of the section 29 of the tube 1 is expanded to increase the volume of the tube. Since tube 1 is solvent filled, after the previous dissolution cycle a small air bubble marker is sucked into probe 2, as shown in FIG. 3. It is obvious that any device, such as a piston, driven by a solenoid, a membrane box, and so on, triggered by an instantaneous increase in the volume or capacity of the combination tube 1, probe 2, can be
    used to introduce such a marker. The volume of the intake air marker 36 should have a very small volume sufficient to seal the tube 1. Accordingly, during the suction cycle, the air bubble is displaced along probe 2 and tube 1 with suction fluid. With a graduated tube 1, the displacement of the air bubble along the tube is an indicator of the volume of the sample, which was all san. For example, portions 37 and 38 of tube 1 are made of a transparent material and correspond to the location of the bubble marker 36.
    Light source 39 is located
    5 to illuminate the transparent portions 37 and 38 of the tube 1. In a preferred embodiment, the length of the portions. 37 and 38 is equal to the length of the air marker 36.
    FIG. 4 and 5 show normal suction cycles of samples of LE and 50X
    0, respectively, with the tube and probe facing upward with respect to parts 37 and 38, respectively, filled with the sample fluid, and the marker
    36 is located in parts 37 and 38,
    5, respectively. The location of the air marker 36 in other parts than
    37 and 38, is evidence that the suction volumes differ from those of samples 10 X or 50 1.
    0 In addition, the location of the blown marker at the input of the probe 2, as shown in FIG. 6 is evidence of the absorption of a short sample.
    five
    The proper location of the air marker can be reliably determined using conventional optical tools or other equipment. For example, an optical device is shown for
    0 determining the presence of an air bubble (marker) in part 37 or 38 and at the same time at the inlet of probe 2, which provides a signal whenever a wrong sample volume is absorbed,
    5 which is necessary. The transmitted light is detected by the detectors 40 and 41, respectively. In addition, the light source 42 is placed to illuminate the transparent input portion of the probe .2,
    0 wherein the transmitted light is detected by the detector 43.
    As is well known, a pipeline that has transparent walls, when it is filled with a light-transmitting fluid,
    5 will act as a cylindrical line, on the other hand, the same pipeline filled with air acts as a diffuser of light due to the inconsistency between the respective air refraction indices and
    0 material that forms the walls of the pipeline, as well as due to the curvature of these walls. Accordingly, when the marker is located between the light source and its corresponding detector
    rum, the light falling on this detector is much larger than the light falling when, these parts are filled with liquid. From this point of view, the characteristics of the detectors 40 and 41, as well as the detector 43, are determined in such a way that they are excited only when there is a liquid between them and the corresponding light sources.
    The output of the detector 43 is connected to the input of the converter 44. The output of the detectors 40 and 41 is connected to the input of logic circuits OR 45 and 46, respectively. Converter output
    44 is connected to the remaining inputs of the OR circuits 45 and 46, respectively. Logic Outputs OR
    45 and 46 are connected to terminals 47 and 48, respectively, of the switch 49. The pole 50 of the switch 49 is selectively connected to terminal 47 or 48 using the programmer 26, which must be cleaned and metered samples of 10 A or 50 L, respectively. Pole 50 is connected via actuation valve 51.
    to the signal circuit 52. The operation of the valve 51 is resolved by the programmer 26 at the conclusion of each suction cycle and before the dosing cycle.
    The presence of marker 36 in part 37 or 38 of tube 1 and the corresponding presence of fluid at the inlet of probe 2, which induces proper suction of 10/1 and 50 I samples, does not trigger the signal circuit 52, and programmer 26 triggers valve 51. In FIG. 4 and 5 show the required locations of air marker 36 in parts 37 and 38, respectively, together with the presence of a fluid sample at the inlet of probe 2 when sucking samples 10 and 50X. For example, in the case of suction of a 10 X sample, detector 41 is energized, while as detector 40 is unexcited. The pole 50 of the switch 49 is connected to terminal 47 by the programmer 26. While the detector 43 is excited, the OR 45 circuit does not work due to the presence of a signal from the converter 44 and the signal circuit 52 does not work, while at the end of the suction cycle by the programmer 26 valve 51 is activated. In the absence of a signal from the circuit 52, programmer 26 gives the command to transfer the sample of liquid to the cell 11.
    If the marker is absent in parts 37 or 38 of tube 1 at the completion of the suction cycle of parts 10 X or 50 I, respectively, at the same time probe 2 is not completely filled with a sample, then, at the permission of the programmer 26, valve 51 is actuated
    the signal circuit 52 is triggered. Similarly, with respect to any suction cycle, the presence of an air bubble at the inlet of probe 2 will excite circuit 45, as a result. That circuit will trigger 52.
    FIG. 6 and 7, the state of the short samples is shown following the suction cycles of the 1 OOD sample, which triggers the signal circuit 52.
    d In FIG. 6 shows the state in which the inlet end 53 of probe 2 is filled with air rather than a fluid sample, and air marker 36 is located in part 37 of tube 1. Accordingly, detector 43 is not excited, and detector 40 is excited. On
    FIG. 7 shows the case of clogging the probe a 2 with debris 54. In this case, the marker 36 is displaced less than in the normal state, while the detector 40
    0 is excited, and detector 41 remains unexcited, and thus the signal circuit 52 is triggered. If the input of probe 2 is littered with debris 54, simulate the presence of airborne
    5 of the marker, such a state allows for the simultaneous excitation of the detector 43. A similar operation takes place with respect to the suction cycle of part 50 L when pole 50 is connected to terminal 48.
    权利要求:
    Claims (2)
    [1]
    Invention Formula
    A device for mixing and dispensing several predetermined reagents, consisting of a probe made in the form of an i-shaped tube with a marker in the form of an air bubble, the sampling end of which is more transparent, a three-channel two-way valve, a connecting tube for sample pumps and reagents solvent source, drives, receivers, cells and
    5 rotary table with drive mechanism and control system, characterized in that, in order to increase the reliability of the device in operation, it is equipped with an input mechanism
    Q marker in action performed from
    sucker and hammer with spring and soleduid, and tracking detectors for the location of the marker, and the connecting tube is made with a section of elastic material with an elastic convex part located between the pillow and the hammer.
    Sources of information taken into account in the examination 1. US patent number 3241432, 23-230, 1966.
    [2]
    2. Patent IT 3971630, 23-230, 1976 (prototype).
    37
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    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    BE627401A|1962-01-23|
    US3419358A|1965-10-19|1968-12-31|Technicon Corp|Automatic analysis apparatus and method|
    US3484170A|1966-04-14|1969-12-16|Technicon Corp|Automatic analysis method and apparatus|
    US3572996A|1967-11-29|1971-03-30|Technicon Corp|Analytical chromatography column monitoring system|
    JPS542618B2|1971-10-19|1979-02-09|
    US3843326A|1972-06-07|1974-10-22|Technicon Instr|Method and apparatus for successive sample analysis without inter-sample contamination|
    US3912456A|1974-03-04|1975-10-14|Anatronics Corp|Apparatus and method for automatic chemical analysis|
    JPS5113677A|1974-07-25|1976-02-03|Kenkichi Tsukamoto|
    US4015938A|1975-11-18|1977-04-05|Technicon Instruments Corporation|Sample supply apparatus and method for automatic analysis systems|FI55910C|1978-01-10|1979-10-10|Suovaniemi Finnpipette|PROCEDURE FOR INSERTING DOSERING AND EXHAUST|
    FR2446480B1|1979-01-10|1985-01-04|Instruments Sa|
    US4318885A|1979-09-10|1982-03-09|Olympus Optical Co., Ltd.|Liquid treating device for chemical analysis apparatus|
    US4253846A|1979-11-21|1981-03-03|Technicon Instruments Corporation|Method and apparatus for automated analysis of fluid samples|
    JPS5681451A|1979-12-07|1981-07-03|Olympus Optical Co Ltd|Separately-injecting nozzle|
    US4399711A|1980-04-18|1983-08-23|Beckman Instruments, Inc.|Method and apparatus ensuring full volume pickup in an automated pipette|
    US4312635A|1980-06-20|1982-01-26|Geochem Research, Inc.|Method and apparatus for fluid analysis|
    JPS5763264U|1980-09-30|1982-04-15|
    JPH0147745B2|1980-12-12|1989-10-16|Olympus Optical Co|
    GB2097692B|1981-01-10|1985-05-22|Shaw Stewart P D|Combining chemical reagents|
    US4399362A|1981-02-27|1983-08-16|Instrumentation Laboratory Inc.|Liquid handling apparatus|
    JPH0134335B2|1981-12-15|1989-07-19|Hitachi Ltd|
    US4419903A|1982-02-22|1983-12-13|Beckman Instruments, Inc.|Method and apparatus for detecting insufficient liquid levels|
    FR2536298B1|1982-11-18|1986-03-28|Boiron Lab Sa|DILUTION APPARATUS, PARTICULARLY FOR THE MANUFACTURE OF HOMEOPATHIC DRUGS|
    JPS643061Y2|1982-11-30|1989-01-26|
    US4476734A|1983-03-07|1984-10-16|International Business Machines Corporation|Wet needle sampler for use with a gas chromatograph|
    JPS6017443U|1983-07-15|1985-02-06|
    US4980292A|1984-10-01|1990-12-25|Baxter International Inc.|Tablet dispensing|
    JPH0627744B2|1984-10-01|1994-04-13|バクスター・ダイアグノスティックス・インコーポレイテッド|Clinical analyzer|
    JPS6347665A|1986-08-14|1988-02-29|Kontron Holding Ag|Method and device for operating pipet|
    US4894345A|1986-12-16|1990-01-16|Ciba-Geigy Corporation|Process for preparation of samples for analysis|
    US4859605A|1986-12-16|1989-08-22|Ciba-Geigy Corporation|Process for preparation of samples for analysis|
    US4900683A|1986-12-16|1990-02-13|Ciba-Geigy Corporation|Process for preparation of samples for analysis|
    US4942018A|1987-02-10|1990-07-17|Ldc Analytical Inc.|Packed bed gradient generator for high performance liquid chromatography|
    US5183765A|1990-10-11|1993-02-02|Drd Diluter Corporation|Means and method of measuring and dispensing|
    US5094961A|1990-12-13|1992-03-10|Coulter Corporation|Aspiration method for hematology analyzing apparatus|
    JPH04295764A|1991-03-26|1992-10-20|Toshiba Corp|Automatic chemical analyzer|
    US5312757A|1991-05-02|1994-05-17|Olympus Optical Co., Ltd.|Sample distributing method|
    DE4203638A1|1992-02-08|1993-08-12|Boehringer Mannheim Gmbh|LIQUID TRANSFER DEVICE FOR AN ANALYZER|
    US5297431A|1992-06-01|1994-03-29|Thermo Separation ProductsInc.|Automated sample dilution|
    US6083762A|1996-05-31|2000-07-04|Packard Instruments Company|Microvolume liquid handling system|
    US6348354B1|1998-07-06|2002-02-19|Bayer Corporation|Method and apparatus for controlling a stream of liquid test packages in a capsule chemistry analysis system|
    DE19919135A1|1999-04-27|2000-11-02|Basf Ag|Method and device for applying small amounts of liquid|
    US6902938B1|2000-10-10|2005-06-07|Jeol Usa, Inc.|Chemical analysis method for multiplexed samples|
    US6579724B2|2001-09-13|2003-06-17|First Ten Angstroms|Dispensing method and apparatus for dispensing very small quantities of fluid|
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    US9562897B2|2010-09-30|2017-02-07|Raindance Technologies, Inc.|Sandwich assays in droplets|
    WO2012057111A1|2010-10-27|2012-05-03|株式会社日立ハイテクノロジーズ|Automatic analysis device|
    EP3412778A1|2011-02-11|2018-12-12|Raindance Technologies, Inc.|Methods for forming mixed droplets|
    WO2012112804A1|2011-02-18|2012-08-23|Raindance Technoligies, Inc.|Compositions and methods for molecular labeling|
    EP2714970B1|2011-06-02|2017-04-19|Raindance Technologies, Inc.|Enzyme quantification|
    US8841071B2|2011-06-02|2014-09-23|Raindance Technologies, Inc.|Sample multiplexing|
    US8658430B2|2011-07-20|2014-02-25|Raindance Technologies, Inc.|Manipulating droplet size|
    US9944977B2|2013-12-12|2018-04-17|Raindance Technologies, Inc.|Distinguishing rare variations in a nucleic acid sequence from a sample|
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    US10647981B1|2015-09-08|2020-05-12|Bio-Rad Laboratories, Inc.|Nucleic acid library generation methods and compositions|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    US05/839,146|US4130394A|1977-10-03|1977-10-03|Short sample detection|
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